Friday, July 14, 2023

Nutrition Scientists Aiding The Nutritionist in Knowledge

 

In the last article on posted only last night on:

The Profession of Nutrition vs The Role of Food Scientist in Research and in Industry

https://scientificlogic.blogspot.com/2023/07/the-profession-of-nutrition-vs-role-of.html

I briefly went through all the various sciences that touched on food and nutrition.

I mentioned the practice of nutrition is now a regulated profession like medicine, and like a doctor who does not specialize, he works as a general medical practitioner, a jack of all trades, master of none. The same is said of a nutritionist whose university training in this nutrition is very broad-based. He knows in some depth the chemistry of foods, biochemistry and metabolism, food analysis, food microbiology, food toxicology, basic medicine with specialization on nutrition deficiency diseases and their diagnosis. He also studies how food consumption is conducted, how nutrition status is assessed using a wide battery of measurements. He has to deal with a wide range of subjects just like a medical student who has to deal with a wide range of diseases, their diagnosis and management.

Armed with a wide range of training he works as a nutritionist, jack of all nutritional trades, master of none. His training is so broad-based that he can fit into any job besides being a nutritionist. He gets all his knowledge from the nutrition and or food scientists who conducts in depth studies on the composition of foods which may or not only necessarily include only protein, fats and carbohydrates sometimes referred to as proximate principles, how they are oxidized in the body to yield energy to meet body needs, but other components of foods.  It may not necessarily include only vitamins, minerals and trace elements but also other bioactive principles that have medicinal and therapeutic properties.

A nutritionist will also have training in the analysis of foods containing these proximate principles as well as vitamins and minerals to establish the food composition table. For instance, he may decide to weigh a small amount of food, determine its protein content by using the Kjeldahl method.  Most proteins contain 16% of nitrogen, thus the conversion factor is to multiply the nitrogen content by 6.25 to obtain the protein content. 

However, the nitrogen from nonprotein additives or contaminants in the food, such as melamine in milk, is also measured. He may then use the traditional Soxhlet extraction of oil using petroleum ether for the extraction of fats and oils. He then subtracts the protein and the fats from the total weight to give the carbohydrates, collectively known as “proximate principles”, ignoring the much smaller amounts of vitamins, minerals and trace elements in them.

If his training in analytical chemistry is advanced enough, he may even use multidimensional gas chromatography, or even using the nuclear magnetic resonance (NMR) analyser to elucidate the organic structure of the other non-food but bioactive principles in them, collectively known as the phytochemicals.

It all depends on the nutritionist level of training in analytical chemistry and the university where he was trained.

He may then proceed to determine the caloric value of the food by using the bomb calorimeter. For vitamins there are a wide range of analytical methods for each of them available to him. For example  in determining potassium, sodium, calcium and other metallic elements, the nutritionist has at his disposal the atomic absorption spectroscopy. The nutritionist as well as the food scientist is well-trained in food analysis as far as the proximate principles (carbohydrates, proteins and fats) is concerned as with the rest of the nutrients.

But over the years nutrition and food scientists have discovered more and more other food principles such as the tens of hundreds of thousands of phytochemicals especially in plant-based foods other than the proximate principles, vitamins and minerals not known previously to a practising nutritionist, let alone his ability to detect and to analyse their composition.

He may only be able to study their effects on the human body both for their preventive and curative properties, but he may not know how they work. These are called functional foods beyond their nutritional values. They have medicinal and therapeutic properties that even dieticians have little training and knowledge. Their analysis and detection are beyond the expertise of both the nutritionists and the dietician. They need to call in the analytical chemists who are armed with their wide range of analytical procedures. These nutrition and food scientists use a wide range of analytical procedures such as the use of spectroscopy meters that include infrared (IR) spectroscopy, far-infrared spectroscopy,  ultraviolet-visible (UV/Vis) spectroscopy, Raman spectroscopy, including nuclear magnetic resonance (NMR) spectroscopy, x-ray spectroscopy, let alone various types of chromatography such as gas chromatography, high-performance liquid chromatography, thin-layer chromatography, and paper chromatography to do the analysis.

Using these analytical procedures are beyond the training, skills and expertise of a practicing nutritionist or a dietician just as much all the advances in medical sciences like genomic and molecular medicine, immunology, virology, Darwinian medicine, radiochemistry, etc, etc are far beyond the knowledge of a medical doctor who is basically a clinician doing clinical work. A clinician often uses lab support for a definitive diagnosis, and these supports are given to the clinician through the knowledge of biomedical sciences.

In order to practise advanced medicine a clinician needs the qualified expertise of the medical scientist to help them with the diagnosis other than merely taking medical history, clinical examination such as palpation, percussion and auscultation with a stethoscope. These include blood and urine analysis or by radiological imaging.  

But if a nutritionist intent to investigate the effects of food on health and disease beyond what they already know on nutritional deficiency diseases, they need the support of the clinician, nutrition scientist, the food analytical chemist, the statistician to design the study, epidemiologists perhaps to look at disease patterns before and after the study.  

They may include animal and laboratory studies, case-control, longitudinal cohort studies and randomized feeding and clinical studies and epidemiology studies.

Cohort Studies:

A cohort is a group of people, so cohort studies look at groups of people. A cohort study follows the group over a period of time. 

A research team may recruit people who may or may have a certain disease under investigation. They collect all available information about them for a number of years. The researchers see who in the group develops a certain disease, and who doesn’t. They then look to see whether the people who developed a disease had anything in common.

Cohort studies are very useful ways of finding out more about risk factors. But they are expensive and time consuming. They can be used when it wouldn’t be possible to test a theory any other way. 

Case Control Studies:

Case control studies work the opposite way to cohort studies. The research team recruits a group of people who have a disease, and a group of people who don't as controls. They then look back to see how many people in each group were exposed to a certain risk factor. 

Researchers want to make the results as reliable as possible. So, they try to make sure the people in each group have the same general factors such as age or gender.

Case control study may show that people with a lower income are more likely to develop a certain disease. But it doesn’t mean that the level of income itself causes the disease. It may mean that they have a poor diet or are more likely to smoke. In clinical and medical research there are many types and classes of studies. They depend on our study design. Just to give examples of some of them are, besides lab studies involving the use of animal models as well. 

Case control studies are useful, and they are quicker and cheaper than cohort studies. But the results may be less reliable. The research team often rely on people thinking back and remembering whether they were exposed to a certain risk factor or not. But people may not remember accurately, and this can affect the results.

Another issue is the difference between association and cause. Just because there is an association between a factor and a disease, it doesn’t mean that the factor causes the disease. In humans we have:  

Cross Sectional Studies

Cross sectional studies are carried out at one point in time, or over a short period of time. They find out who has been exposed to a risk factor and who has developed a certain disease. They see if there is a link. 

Cross sectional studies are quicker and cheaper to do. But the results can be less useful. Sometimes researchers do a cross sectional study first to find a possible link. Then they go on to do a case control or cohort study to look at the issue in more detail.

Longitudinal Studies:

Then we have longitudinal studies involving cohorts designed to discover relationships between variables that are not related to various background variables. This observational research technique involves studying the same group of individuals over an extended period. An example is the Framingham Heart Study which is a longitudinal cohort study that started since 1948 and has been followed up among cohorts over the generations. That study is where we get our idea about cholesterol and heart disease. But as that study was followed up over the years the same Framingham Heart Study withdrew their same cholesterol and heart theory, but the Big Pharma that produces those statins group of drugs to lower down blood cholesterol levels did not wish to listen as it was a multi-billion-dollar industry for them.

All these new discoveries and new knowledge are far beyond a nutritionist or a medical doctor to accept. They stick on to old textbook knowledge.

Nutrition research to evaluate just one component of food on health and disease is a teamwork effort utilizing the expertise from various disciplines of sciences. They may include anthropometry, sociology, food and nutrition sciences, food consumption studies, food toxicology, medicine, chemistry, biochemistry, food analysis, clinical examination, among a host of others depending on the nature and scope of the study. They all have to work together in coordination as a scientific and medical team.   

Intensive and Extensive Research: 

Nutrition research is far from being easy. It is not like practising medicine or practicing nutrition, or dietetics.  

I have much, much more to explain, but we shall give just a very brief look at the scope of nutrition research. In fact the research expenditure for nutrition is the highest among all the medical disciplines, amounting to over US $ 100 billion a year globally resulting in an avalanche of new knowledge published in tons of research papers on nutrition each year, and yet we have so much to learn the impact of food and nutrition in the prevention and treatment of all these chronic lifestyle, especially dietary lifestyle diseases which plague the affluent society globally  for which we have no definitive answer.

Nutrition is so complex and confusing that health companies and individuals every now and then make health claims that this and that food can cure cancer, blocked arteries, neurological diseases and stroke, arthritis, kidney, liver and lung diseases for which even the most qualified nutritionist or a nutrition researcher has no clue. They merely claim with an impressive list of foods that can ‘cure’ this and that disease without showing any evidence that can only come about from any well-designed and well controlled study. They just make personal claims from personal experience, all mixed up with the medical treatment they were already receiving.

Health and Labelling Claims:

Then we also have health food manufactures making all sorts of health claims about their products and label them as a cure so much so, nutritionists are very alarmed. So, nutritionists as health professionals set up committees to advise the Ministry of Health and other legislative authorities to enact food laws on health and labelling claims on their products.

Food Additives:

The same, nutrition scientists and food toxicologists conduct studies on food additives, food colours, food flavours, food preservatives on the safety and long-term toxicity of some of these food substances added to the food by manufacturers to enhance their acceptability, keeping quality for commercial reasons. These scientists conduct costly and long-term toxicological studies on these food additives to advise the nutritionists their effects on health. The nutritionists in turn advise the government the maximum limits these additives are allowed for safety. The government in turn enact food laws such as the Food Act 1983 and the Food Regulations 1985 to ensure that the public is protected from health hazards and fraud in the preparation, sale and use of foods and for matters connected therewith. It is then enforced by the Ministry of Health and the Local Authorities.

Personal vs. Evidenced-Based Claims:

For instance, a few days ago I received a video claim that was going round in the social media from a male patient suffering from cancer who did not even specify what type of cancer. He claimed that papaya leaves had cured him of his cancer even though he received chemotherapy at the same time. So, we do not know if it was the chemotherapy or the papaya leaves that caused his cancer to ‘disappear’ as he claimed when even highly qualified nutritionists and oncologists have no clue. How do we deal with such problems from the public is very difficult for me to understand.

Just this morning I met a gentleman at the bus stop who was a stranger to me. He saw my leg all wrapped up in compression stockings. He asked me what happened to my leg. I explained to him I have chronic venous stasis ulcers, and the mechanisms why and how this happens. He took out a small label of some herbal remedy which I can buy from the Chinese medical hall. He told me this herb will solve my problem. Of course, I did not offend him that it will not work because mine is a mechanical (surgical) problem best managed surgically and physically. I thank him of course for his suggestion.

We can see lay people make all kinds of claims without showing the thinnest of evidence let alone large scale RCT (randomized clinical trials), case control studies, cross sectional or longitudinal cohort studies I mentioned above. But at least there must be some minimal evidence serving as a springboard for large scale studies.  For instance, as described below about cukur manis.

Papaverine in Cukur manis As Evidence-Based:

Let me now illustrate an example. In the beginning of 1970’s one of my Readers in Nutrition (Senior Lecturer / Associate Professor as they were called in the UK) from the University of London came over to Malaysia at the invitation of the National University of Malaysia (UKM). UKM wanted to establish the Faculty of Medicine here in Kuala Lumpur. My Reader was invited as a consultant to UKM on how to go about this.

I met him when one of the professors from UKM contacted me. I invited my Reader from London for lunch and during our luncheon conversation he mentioned about one of the staff from UKM who went to London University to do a postgraduate course in nutrition like I did in the early 1960’s. He told me this particular student told him (my Reader) that there were reports from villagers here in Malaysia having fainting spells when they ate a certain vegetable. My Reader asked this student, who is a doctor, to send samples of this vegetable to him.

The doctor from UKM did. They at London University identified it as Sauropus androgynus which is cukur manis in Malay.

But why and how did cukur manis cause fainting spells? Nutritionists at London University did not know. So, they sought the help of their colleagues, the analytical chemists working in the Department of Chemistry of the same college (Queen Elizabeth College) to analyse what inside cukur manis.

So, the chemists from the Dept of Chemistry at Queen Elizabeth College did by using their powerful Nuclear Magnetic Resonance (NMR) spectroscopy. They identified the compound present in the cukur manis as papaverine, and it was this that caused the fainting spells among the villagers.  

Papaverine is an opium alkaloid, primarily used as an antispasmodic drug and as a cerebral and coronary vasodilator. It has vasodilating on the blood vessels, causing the blood pressure to fall when taken orally, and hence synoptic (fainting) effects of papaverine.  It was a drug used in the Mediterranean to treat high blood pressure. There you are. The analytical chemist had used their discovery to provide knowledge to the nutritionist to explain the cause of the fainting. The nutritionist would know this if the chemist had not analysed the unknown compound in the vegetable.

In turn the nutritionist can advise individuals with high blood pressure to consume cukur manis in small amounts for this medicinal effect. 

However papaverine can also cause serious side effects such bronchiolitis obliterans and damange to the lungs if consumed in large amounts. But if cooked properly it seems safe for consumption since it is also very rich in provitamin A, the carotenes, vitamin B and C, protein and minerals especially iron. It is probably one of the best vegetables nutrition and medicinal-wise in moderation.   

The nutritionist in turn would pass this knowledge to the practising dietician to prescribe diets containing natural papaverine that can lower down high blood pressure, increase blood flow and not just lowering down their salt (sodium) intake to reduce blood pressure if consume in moderation. 

This is just one example we learn that foods too have therapeutic properties, not just nutritive values. Most fruits and vegetables, especially highly coloured ones in the tropcs have very high medicinal and antioxidants properties, not just their provitamin A and vitamin C contents. 

We can clearly see how scientists from different disciplines have help the nutritionist advance their knowledge with their discoveries to help each other. They then pass on their discoveries and knowledge to  help society. We can see how this single, tiny bit of discovery on this vegetable as an example can serve as a springboard for larger scale randomized clinical studies. Each scientist in the research team contributing their wealth of scientific expertise to their research colleagues.We cannot work alone as if we are the only expert in the entire world. We need the expertise of our colleagues working in another field to help us when we do not know. We cannot be arrogant and self-centred. Even in research we work as a team. We cannot work alone. We form a committee, a team of experts from different disciplines of sciences or medicine sharing our ideas together to solve problems together. They may have better ideas than us. To claim we know better is morally, socially, ethically distasteful to a decent society.  

Food as Medicine: 

The same we may say of individual and traditional claims that certain foods have medicinal and therapeutic properties. Curative properties are not just drugs and medicines from pharmaceutical companies.

Some 2,300 years ago the Greek physician Hippocrates, considered as the Father of Medicine, already told physicians during his time “Let Food be Thy Medicine”. He never said let medicine be thy food? This was put into his mouth long, long after his death by drug companies, probably by doctors too influenced by the very powerful and influential pharmaceutical companies in the west and in the United States to promote their drugs as medicines instead of food.

We never know which foods, especially all the highly coloured fruits and vegetables, have medical properties, not just nutritive values since we are dealing with tens of thousands, if not hundreds of millions of phytochemicals in plants. There is no way for any nutrition scientist to help the nutritionist to screen all those untold numbers of fruits and vegetables.  

Nutritionists now label them as “functional foods” – foods that serve beyond just nourishing the body – foods that serves as medicines for tens of thousands of years since the time of Adam and Eve in the Garden of Eden where God told them they may partake from every tree of knowledge in that Garden that would have given them health and eternal life, except one tree, the tree of evil. There is a herb for every illness on earth

Does that message ring the bell?   

Nobel Prizes in Medicine for Scientists None for Doctors:    

It is no surprise that all the Nobel Prizes in Medicine or in Physiology went to the medical scientists rather than to the medical doctor since 1927. It was the scientists in biomedical sciences who contributed almost 99.9 % of their discoveries and knowledge to the medical doctors and nutritionists to educate, practise and apply them on their patients.  

I think I need to stop here to rest, else it never ends. It will run into chapters all through the night till dawn into days should we continue to write.

We can learn from each other, as much as a doctor or a nutritionist can learn from other biomedical scientists.  

Thank you for reading.

Lim ju boo   

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