Sunday, February 21, 2021

How Safe is mRNA Vaccines?

 

How safe is mRNA vaccine?

 

lim ju boo

Post-doctoral CE Evolution (Cambridge)

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·         Viruses go through evolution by natural selection similar to all life forms. However viruses are able to mutate much faster and hence capable of evolving into much more strains and variants at unprecedented speeds compared to more complex organisms such as bacteria.  

·         There is likelihood that other viruses may also infect a cell simultaneously. When that happens they may switch genetic material to construct new type of viruses with distinctive behavior.

·         Mixed new strains and variants of SARS-CoV2 virus may arise through multiple mutations, and introduced into the body via compounded infections

·         In mixed infections they give  rise to different clinical presentations and complications

·         It is well-known that RNA viruses have very rapid mutation rates that allow it for fast evolution into numerous strains and variants. This may cause resistance to drug or vaccines due to changes in their genomic profiles and behavior.  

Through exchanges in their gene pool from mixed virus population it can rapidly evolve over time through natural selection into highly infectivity

One reason why virus such as SARS-CoV2 is able to mutate into various variants very rapidly is because of the simplicity and shorter RNA chains of about 30 kilobits.

By random mutations of changing their RNA sequence or codes new strains and new behavior of the subsequent variants arises

 

Origin of Viruses:

                        

There are many theories forwarded on the origin of viruses, probably including the corona group of viruses such as one given here;

https://www.nature.com/scitable/topicpage/the-origins-of-viruses-14398218/#:~:text=Viruses%20may%20have%20arisen%20from,the%20evolution%20of%2C%20cellular%20life.

We shall look at this theory in another way later

However the origin of the corona virus that causes the severe acute respiratory syndrome (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV) are two highly transmissible and pathogenic viruses.

It is believed to have surfaced in humans at the beginning of the 21st century, and they are highly transmissible and pathogenic viruses

Many theories and conspiracies have emerged on the origin of the SARS-CoV2 group, among them  were thought to be from bats, pangolins, civet cats and even politically suspected to be artificially created by the Chinese in Wuhan, China

But then the genetically diversity or even similarity of the corona viruses related to SARS-CoV and MERS-CoV discovered in bats worldwide did not suggest this group of viruses were artificially created, or  have recently evolved.

Our current understanding on the origin and evolution of these two pathogenic corona viruses is that it may be a spillover from bat-borne coronaviruses, or from swine acute diarrhoea syndrome coronavirus (SADS-CoV) to pigs.

But going back to the theory on the origin of all viruses, it may have been the result of untold numbers of mutations from the first chemistry of RNA which may have taken place 3,800 million years ago by random combination of nitrogen compounds and nucleotide floating in the warm primordial oceans after Earth was created.

See list of Evolution of Life on Earth here:

https://scientificlogic.blogspot.com/             

Please also refer to Ir. Lau TO blog on the myriads of ways the Covid-19 virus could change their genetic combinations  here:

https://taionn.blogspot.com/ 

It is claimed that this novel vaccine is made from Messenger RNA (mRNA). What does that mean?

mRNA from  SARS-CoV2 is a molecular fragment of the virus  that carries the codes for  protein synthesis.  For instance, the message from mRNA is for the formation of their  spike proteins

mRNA was first described in 1956 by Elliot Volkin and Lazarus Astrachan. In addition to mRNA, there are two other major types of RNA: ribosomal RNA (rRNA) and transfer RNA (tRNA) that may be present in other cells, but we shall not go into them.  

According to Biontech this is how mRNA is made and how the vaccine works.

First, briefly explained, mRNA is synthesized by using the nucleotide sequence of DNA or RNA as a template. This procedure requires nucleotide triphosphates as substrates and is catalyzed by the enzyme RNA polymerase II. The process of making mRNA from DNA or RNA is called transcription,

Messenger ribonucleic acid (mRNA) vaccines are then made from this new process that is believed to be able to stimulate the body’s own immune response. These vaccines carry orders from the messenger RNA, including the genetic copies or code of a specific virus trait (virus antigen). The information allows the body to produce the antigen on its own.

The mRNA reassigns the information for the production of the antigen to the cell machinery that expresses the proteins. Cells in our body then present the antigen on their surface, and thus it activates the preferred specific immune response.

 

When the body comes in contact with the virus, the immune system recognizes the specific antigen. It then wrestles the virus in a targeted manner.

The entry of the COVID-19, into the host cell in humans involves the coronavirus spike protein that is located on the surface of the virus.

 

mRNA vaccines against COVID-19 are intended to provide our bodies with the code to produce the non-infectious virus according to the manufactures claim

 

The spike proteins instruct the cell’s machinery help stimulate a natural immune response. This response is also achieved principally through T-cells

 

Once a person is vaccinated and later comes into contact with SARS-CoV-2, the immune system will recognize the surface structure and will be able to combat the virus and destroy it.

 

Neutralizing antibodies targeted at SARS-CoV-2 circulating in the body will immediately bind to the virus by “neutralizing” it and inhibiting it from entering the cell

 

The T cells simultaneously also assist the immune system to fight intracellular infections and can also destroy the infected cells directly.
 
Thus, according to the manufacturer’s claim, unlike conventional vaccines, a mRNA vaccine does not contain any viral proteins itself, but only the information from the synthetic mRNA that our own cells need to produce a virus trait that triggers the desired immune response.

But the question we also need to ask is, if the vaccine merely mimics the surface structure that is synthetically-produced, and hence it is also bio-similar to the real virus

In such a scenario, wouldn’t this molecular fragment over time becomes biologically active into the real virus?

This would be very similar to how viruses first evolved other than the theories give above.

The supposedly nitrogen compounds or base plus sugars - pentose sugar (5-carbon sugar) and the phosphate group, that may have been floating in the warm primordial oceans between 3,800 to 4,000 million years ago may have formed the first nucleic acids, and subsequently integrated into nucleotides chains that then evolves into  real viruses. These compounds were pre-life as precursors to the real virus to be evolved later

It may seem to take a long, time under the natural environment where no other host were present 4 billion years ago.  But to my understanding, this may not be the case where a human host is already available right now for it to immediately hijack and evolve into a real virus exceedingly rapidly especially with a lot of human genomic materials to help it, exactly like bacteria being found to be very capable of incorporating stray DNA from the environment into their own for them to evolve into better and hardier strains. This is the real risk we need to consider.   

This is the same analogy with drugs that exerts the same pharmacological effect on the body, whether they were the original patented drugs or their generic bio-similars since their molecular structure, configuration and mass of both patented and generic are exactly the same. Both exert the same effect on the body.

In short, it to my mind, it makes no difference if the mRNA vaccine was synthetic or extracted from the real Covid-19 virus because their molecular similarity

I think this is the real risk no one so far is able to answer or for sure.

Logically, I have my personal reservations with this hardly-studied technology   

 

 

 

 

1 comment:

Anonymous said...

Great Sifu,

Just testing if I post a comment on your blog. Lau

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